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Shiitake and porcini may be more than culinary delights.

Gourmet Medicine: Edible Mushrooms May Help Fight Cancer

by Jennifer Amie

Shiitake and porcini mushrooms are prized by cooks, but they may provide much more than good flavor. For centuries, the healing properties of mushrooms have been recognized by practitioners of traditional medicine in China, Japan, and Europe. Today, Bell Museum curator of fungi David McLaughlin, graduate student Bryn Dentinger, and Dr. Joel Slaton of the University of Minnesota Medical School have teamed up to investigate the cancer-fighting properties of these delicacies.

"In Europe, porcini have been used as a cancer treatment since the Middle Ages, and they've been used in Chinese medicine for much longer than that," says Dentinger.
Evidence from a 1950s study at Memorial Sloan-Kettering Cancer Center supports the theory that porcini may be quite potent. "The study showed 80- to 100-percent inhibition of two different tumors with extracts of Boletus edulis [porcini]," Dentinger explains.
Dentinger is providing Slaton with dried tissue from two types of porcini mushroom: one that grows on oak trees in Minnesota and a second that grows on West Coast conifers.
Slaton tests extracts from these samples on cancer cells grown in the laboratory and on tumors in mice. If the extracts shrink the tumors, Slaton will try to identify the compounds that inhibit cancer growth.

Slaton is also studying shiitake mushrooms, which produce immune-boosting vitamin D.
For these laboratory tests to be meaningful, Slaton must know exactly which mushrooms he is testing. Shiitake mushrooms from Japan, for example, may be very different from shiitake mushrooms from Southeast Asia, Dentinger says.

Researchers not only need to know which type of mushroom they're using in experiments, but must also be sure they're using the same type from day to day—and therein lies the problem.

"What we think of as 'porcini' may in fact be as many as 39 different species," says Dentinger. "Though they look alike, they may not be closely related."

Porcini cannot be cultivated and must be collected from the wild, further complicating the task of identifying the mushrooms correctly and consistently.

For his part, Dentinger is trying to untangle the genetic relationships among the various mushrooms known as "porcini."

Porcini grow throughout the temperate zone of the northern hemisphere, from Asia to Europe to North America, and are found as far south as Yunnan, China, Himalayan India, and Costa Rica. "Everybody's using different names for these mushrooms," says Dentinger.
Scientific names, of course, are meant to dispel such confusion by creating a universal standard. The first time any species is identified and named by a scientist, a specimen of that species—whether bird or fish or mushroom—is preserved in a museum or herbarium. Researchers can always return to this original specimen, known as a "type specimen," for comparison when they are trying to identify a species.

The scientific name for porcini is Boletus edulis, and it just so happens that its type specimen is a painting. The herbarium that contained the actual mushroom was destroyed during the French revolution, when the botanist who named the species was killed under mysterious circumstances. The botanist's painting of Boletus edulis (known as an iconotype) is believed to survive in the collections of the Muséum National d'Histoire Naturelle in Paris, and Dentinger is trying to track it down. In the meantime, all he has to go on is a reproduction of the painting published in 1791 in Pierre Buillard's Histoire des Champignons de la France (see cover photo).

Unfortunately, the illustration contains no details about the color, size, and shape of the mushroom's spores and cells—all elements critical to proper identification of mushrooms.

While this puts Dentinger at a disadvantage as he attempts to sort out which of his mushroom specimens are truly Boletus edulis and which are not, he does have a modern trick up his sleeve: genetic analysis.

Dentinger is examining DNA from porcini specimens in museums around the world, as well as from mushrooms he's collected himself.

His goal is to extract and sequence DNA from all the various types of porcini to sort them into species groups. He will then be able to determine which groups are most closely related—an important factor for biochemists trying to isolate drug compounds. If a mushroom is found to have cancer-fighting properties, scientists might also look to its closest relatives for medicinal benefits.

Dentinger believes that porcini and shiitake mushrooms may be most helpful as an ingredient in our diets. "It's not clear whether they contain something that you could synthesize as a chemotherapy agent, or whether you could incorporate these mushrooms into your diet and get a protective benefit," he says. "I think their greatest potential is as delicious edible mushrooms."



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